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Many studies have shown that DNA methylation is an important epigenomic mechanism involved in various biological processes, regulating gene expression and chromatin dynamics. Whole genome bisulfite sequencing (WGBS) has proven as a useful technique for studying genome-wide DNA methylation in mammals. However, WGBS data analysis procedure is usually complicated and challenging. To alleviate the analytic difficulties, we integrated the upstream read quality and adapter trimming, bisulfite alignment, methylation calling and methylation measurement steps into Galaxy platform and developed EpiMOLAS system for versatile WGBS data analysis and visualization. Through this customized Galaxy Docker container platform (DocMethyl) and EpiMOLAS web-based system, users can rapidly deploy an execution environment and perform reproducible data analysis with various research scenarios.
EpiMOLAS is available in http://symbiosis.iis.sinica.edu.tw/epimolas. Present available reference genomes in epiMOLAS includes human (GRCh37/hg19, GRCh38/hg38), mouse (GRCm38/mm10) and Arabidopsis (TAIR10).